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Biotinylated anti-mouse secondary antibody Normal goat serum, 4% in PBSA Bovine serum albumin (BSA), 3% in PBSA Neutral buffered formalin, 10% EtOH PBSA Paraf n wax Microtome Protocol (a) Remove vessels from bioreactor by cutting the silicone tubing at both ends near the glass sidearms so that the vessel is removed with the silicone tubing retained in the lumen. Immediately x in 10% neutral buffered formalin for 1 h. Fixing the vessel with the silicone tubing still in the lumen prevents shrinkage and subsequent reduction of the size of the vessel lumen. (b) Dehydrate samples in EtOH and embed in paraf n wax according to standard histologic procedures. (c) Prepare vessel cross sections of 4- m thickness with a microtome, and dewax according to standard histologic procedures. (d) Block with 4% normal goat serum and 3% bovine serum albumin (in PBSA). (e) Stain for the presence of SMC-speci c proteins, using the anti-smooth muscle -actin (diluted in PBSA 1:500) and anti-calponin (diluted in PBSA 1:10,000) antibodies. EC-speci c proteins are stained for with the anti-von Willebrand factor antibody (diluted in PBSA 1:500). (f) Visualize antibody-antigen complexes with the Vectastain Elite ABC Kit and the included biotinylated anti-mouse secondary antibody (diluted in PBS 1:250) according to the manufacturer s protocol.

where MA is the molar mass of the solvent (grams), mi the molal concentration of solute species i and the summation is over all solute species. For systems in which water is the only solvent, this reduces to f B xwater = B Thus, the new expression for A would become A = + RT ln( A mA ) A (6.21) where is the new standard state according to the new scale. Thus, non-ideal solutions A approach ideality when the concentrations of all components but one approach zero. In that case, the solutes satisfy Henry s law where the solvent satis es Raoult s law. An aqueous aerosol solution droplet approaches ideality as it becomes increasingly dilute; that is, the composition approaches that of pure water. It is also important to recognise that the activity coef cient is a parameterisation of very complicated thermodynamic behaviour and it is the modelling of such behaviour that provides a great challenge in attempting to represent real behaviour in the aerosol particle. For solids, the activity is simply 1, and for gases it is de ned as the partial pressure divided by 1 atmosphere. Indeed, the chemical potentials for solids and gases can be de ned, respectively, as A (s) = A A (g ) = + RT Ln(aA ) = + RT Ln A A

One of the ways to keep an eye on the accounts receivable is to periodically (possibly once a month) prepare a chart showing the age of the accounts outstanding. Exhibit 11.7 illustrates such a chart and shows that the accounts receivable outstanding situation has not improved from March to April. In March, 79.5 percent of total receivables were less than 30 days old. In April, only 74.2 percent were less than 30 days outstanding. Similarly, the relative percentages in the 31- to 60-day category have worsened from March to April. As well, in the 61- to 90-day bracket, 11.3 percent of receivables are outstanding in April, against only 3.2 percent in March. This particular aging chart shows that our accounts receivable are getting older. If this trend continued, collection procedures would need to be improved. If, after all possible collection procedures have been explored, an account is deemed to be uncollectable (a bad debt), it

European R-2 Signaling System Frequencies (Hz)

The main user interface for Spotlight is the ubiquitous, blue-encircled magnifying glass at the right end of the menu bar. When users click there, they see the simplest search interface possible: a box to type in (Figure 20-2). As soon as users type in the box, Spotlight begins searching metadata for matches, refining the search as more text is typed. Eventually, the list of results dangles below the search box, starting with a Search All item, followed by Top Hit (Figure 20-2). Every item in the list is clickable. The user can click any item to open it directly in its application. Clicking Search All displays the results in more detail and with filtering options. You can, for example, choose to display only items created or modified today, or you can sort items by author.

In the preceding example, the letter spacing will be decreased by 0.2em for P elements with a class of legal. You can also expand or condense fonts by increasing or decreasing the white space between words. The property for this is called word spacing. It works like letter spacing, which adds to the default amount of white space between words.

The name of the plugin (that is, the value passed to the name method) is used as the plugin name in the stored data, so do not put the version number of the plugin in there.